Introduction: Due to the emergence of antibiotic and multi-drug resistant pathogens, there is a growing need for development of novel and effective antimicrobial wound care technologies. In the last few years, understanding the role of microorganisms living in biofilms has increased. Biofilms are complex colonies of microorganisms encased in a dense extracellular matrix. Biofilms are a major contributor to diseases characterized by an underlying microbial infection and resulting chronic inflammation. Biofilms account for more than 80% of human bacterial infections. Our study was focused on further evaluation of novel anti-microbial gels focused on wound pathogens.
Methods: Antimicrobial and anti-biofilm testing was conducted against bacterial and fungal wound pathogens. Various concentrations of pathogens were tested. Zone of Inhibitions (ZOI) studies and antibiofilm testing were conducted for three novel silver gels against bacterial and fungal pathogens including molds that are of clinical relevance. 200uL of 10^6 CFU/mL of each strain was inoculated onto a TSA plate (bacteria) or PDA plate (fungus and mold). One drop of each gel treatment was placed onto assigned sections for each sample plate. Plates were incubated for at 370C incubator. We conducted efficacy of the test samples against immature and mature biofilms of MRSA and P. aeruginosa.
Results: Our results demonstrated that three novel silver gel formulations have significant anti-microbial efficacy against Acinetobacter baumannii, E. coli O157, Enterobacter cloacae, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, MRSA (Methicillin Resistant Staphylococcus aureus), Candida albicans, Candida auris and Aspergillus flavus and biofilms of MRSA and P. aeruginosa.
Discussion: The candidate silver-based products have significant application for preventing wound infections and the biofilms.
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